Submitted:

•   Integrin and Cell Adhesion Molecules:  Methods and Protocols.Yingchun Wang, Richard Klemke.  Book chapter for the Methods in Molecular Biology.  Immune Disease Institute.  Program in Cellular and Molecular Medicine at Children's Hospital Boston. Department of Anesthesia, Harvard Medical School. Center for Life Science Boston.  Submitted.

• PEAK1 is a novel cytoskeleton-associated kinase that promotes cell migration and cancer progression. Yingchun Wang, Hop S. Tran Cao, Greg T. Cantin, Rui Lin, Wei Wang, Sharmeela Kaushal, Jonathan Kelber, Thomas S. Edgington, Robert M. Hoffman, Michael Bouvet, John R. Yates, Richard L. Klemke.  Submitted to Cancer Cell.

• Allosteric RAAF Kinase Inhibitor Disrupts Angiogenesis by Preventing Endothelial Lumen Formation
  Eric A. Murphy, Wolgang Wrasidio, Konstantin Stoletov, Elena Dneprovskaia, Michel McElroy, Jeff Lingquist, Adrian Sasaldanha, Richard Soll, George E. Davis, Michael Bouvet, Richard Klemke, David A. Cheresh. JCI. Submitted.

• EGFR Regulates Metastasis through an Integrin αvβ5/src Signaling Axis
  Jill M. Ricono, Milan. Makale, Yingchun Wang, L. Barnes, David Schlaepfer, Richard Klemke, David Cheresh. Submitted.

• Structures of PHR domains from Mus musculus Phr-1 (Mycbp2) explain the loss of function mutation of C. elegans ortholog RPM-1.
  Parthasarathy Sampathkumar, Wang Y, Ozyurt A.S., Wassermann S, Miller S, Bain K, Rutter M, Gheyi T, Atwell S, Thompson D, Emtage S, Sauder JM, Klemke R.L. and Burley S.K. Submitted.

Published:

• Chemoattractant signaling between tumor cells and macrophages regulates cancer cell migration, metastasis and neovascularization.
  
Green CE, Liu T, Montel V, Hsiao G, Lester RD, Subramaniam S, Gonias SL, Klemke RL. PLoS One. 2009 Aug 21;4(8):e6713. PMID: 19696929

• Identification and bioinformatic analysis of the membrane proteins of synechocystis sp. PCC 6803.
  Wang Y, Xu W, Chitnis PR. Proteome Sci. 2009 Mar 25;7:11.

• Vascular Lipid Accumulation, Lipoprotein Oxidation, and Macrophage Lipid Uptake in Hypercholesterolemic Zebrafish.
  Stoletov K, Fang L, Choi SH, Hartvigsen K, Hansen LF, Hall C, Pattison J, Juliano J, Miller ER, Almazan F, Crosier P, Witztum JL, Klemke RL, Miller YI.  Circ Res. 2009 April 14;104(8):952-60. PMID: 19265037

• Quantitative Phosphoproteome Analysis of Lysophosphatidic Acid Induced Chemotaxis applying Durl-step 180 Labeling coupled wit Immobilized Metal-ion Affinity Chromatography.
  Shi-Jian Ding, Yingchun Wang, Jon M. Jacobs, Wei-Jun Qian, Feng Yang, Aleksey V. Tolmachev, Eric F. Strittmatter, Ronald J. Moore, Matt E. Monroe, Samuel O. Purvine, Quanzhou Luo, Katrina Waters, David G. Camp II, Richard Klemke, Richard D. Smith. Journal of Proteome Research.  2008 October; 7(10):4215-24.

• Catch of the day, zebrafish as a human cancer model.
  Stoletov, Konstantine, Richard Klemke. Oncogene.  2008 Jul 31;27(33):4509-20.

• The marine lipopeptide Somocystinamide A triggers apoptosis via caspase 8.
  Dwayne G. Stupack, Ainhoa Mielgo, Vicente Torres, Simone Barbero, Konstantin Stoletov, Richard Klemke, William Gerwick, Dennis Carson & Wolf Wrasidlo. PNAS 2008 Feb 19;105(7):2313-8.

• Spatial Mapping of the Neurite and Soma Proteomes Reveals A Functional Cdc42/Rac Regulatory Network
  Olivier Pertz, Yingchun Wang, Feng Yang , Wei Wang, Laurie J. Gay, Marina A. Gristenko, Therese R. Clauss, David J. Anderson, Tao Liu, Kenneth J. Auberry, David G. Camp II, Richard D. Smith, Richard L. Klemke. .  2008 PNAS.  Proc Natl Acad Sci U S A. 2008 Feb 1 [Epub ahead of print] Link to PNAS  
  

• High Resolution Imaging of the Dynamic tumor Cell-Vascular Interface in Transparent Zebrafish
  Konstantin Stoletov, Valerie Montal, Robin Lester, Steven Gonias, Richard Klemke. . PNAS. 2007. 104(44):17406-11.     Faculty 1000 Score 6.0

• PhosphoBlast: A Computational Tool for comparing Phosphoprotein Signatures among Large Datasets.
  Yingchun Wang, Richard L. Klemke. Molecular & Cellular Proteomics. 7:145-162, 2007.

• Methods for Pseudopodia Purification and Proteomic Analysis.
  Yingchun Wang, Shi-Jian Ding, Wei Wang, Feng Yang, Jon M. Jacobs, David Camp, II, Richard D. Smith, and Richard L. Klemke.   Sci. STKE, 2007 (400), p.l4.

• Profiling signaling polarity in chemotactic cells.
  Yingchun Wang, Shi-Jian Ding, Wei Wang, Jon M. Jacobs, Wei-Jun Qian, Ronald J. Moore, Feng Yang, David G. Camp II, Richard D. Smith, and Richard L. Klemke. Cell Biology PNAS 2007. 104(20):8328-33. Faculty 1000 Score 6.0 .

• Biochemical Purification of Pseudopodia from Migratory Cells, Methods in Molecular biology. 
  Yingchun Wang, and Richard L. Klemke (2007). A.S. Coutts, ed. (Totowa, NJ: Humana Press Inc.),
  pp. 55-66.

• Computational Methods for Comparison of Large Genomic and Proteomic Datasets Reveals Protein Markers of Metastatic Cancer.
  Wang, Y., Hanley, R. Klemke, R.L. J Proteome Res. 5(4):907-15. 2006

• Spatio-temporal dynamics of RhoA activity in migrating cells.
  Pertz, O., Hodgson, L., Klemke, R.L. and Hahn, K.M.Nature. 440(7087):1069-72. 2006.

• Deregulation of Proteasome Function Induces Abl-mediated Cell Death by Uncoupling p130CAS and c-CrkII.
  Holcomb, M., Rufini, A., Barilà, D., and Klemke, R.L.. J. Biol. Chem. 281(5):2430-40. 2006.

• R-Ras controls membrane protusion and cell migration through the spatial regulation of Rho and Rac.
  Wozniak M.A., Kwong L., Chodniewicz D., Klemke, R.L. & Keely PJ. Mol. Biol. Cell. 16(1):84-96. 2005.

• Guiding cell migration through directed extension and stabilization of pseudopodia.
  Chodniewicz, D. & Klemke, R.L.Exp. Cell Res. 301:31-37. 2004.

• Regulation of Integrin-mediated Cellular Responses through Assembly of a CAS/Crk Scaffold.
  Chodniewicz, D. & Klemke, R.L.. Biochimica et Biophysica Acta.

• Regulation of Cell Migration and Survival by Focal Adhesion Targeting of Lasp-1
  Lin, Y., Park, Z., Lin, D., Brahmbhatt, A., Rio, M. & Klemke, R.L.  . J. Cell Biol. 165(3):421-432. 2004.

• Regulation of Cell Motility by Abl Family Kinases. In T. Koleske (Ed.), Abl Family Kinases in Development and Disease.
  Emami, S. & Klemke, R.L.(2005)Landes Bioscience. http://www.eurekah.com/abstract.php?chapid=2426&bookid=185&catid=56.

• Novel design for quantification and purification of neurite outgrowth.
  Smith, M. Leng, J. & Klemke, R.L.BioTechniques. 35:254-256. 2003.

• Cytoplasmic c-Abl provides a molecular rheostat controlling carcinoma cell survival and invasion.
  Kain, K., Gooch, S., & Klemke, R.L. Oncogene. 22(38):6071-6080. 2003.

• ERK and RhoA differentially regulate pseudopodia growth and retraction during chemotaxis.
  Brahmbhatt, A. & Klemke, R.L.J. Biol. Chem. 278:13016-13025. 2003.

• Assay for neurite outgrowth quantification. 
  Smit M, Leng J, Klemke RL. Innovative Cell Systems LLC, Temecula, CA, USA. Biotechniques. 2003 Aug;35(2):254-6.  PMID: 12951763

• Purification of pseudopodia from polarized cells reveals redistribution and activation of Rac through assembly of a CAS/Crk scaffold.
  Cho, S.Y. & Klemke, R.L  J. Cell. Biol. 156(4):725-736. 2002.

• Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells.
  Ting, A.Y., Kain, K.H., Klemke, R.L. & Tsien, R.Y. Proc. Natl. Acad. Sci. 98(26):15003-15008. 2001.

• Inhibition of cell migration by Abl family tyrosine kinases through uncoupling of Crk-Cas complexes.
  Kain, K. & Klemke, R.L.J. Biol. Chem. 276(19):16185-16192. 2001.

• Association of p130CAS with phosphatidylinositol-3-OH kinase mediates adenovirus cell entry.
  Li, E., Stupack, D.G., Brown, S.L., Klemke, R.L., Schlaepfer, D.D. & Nemerow, G.R. . J. Biol. Chem. 275(10):14729-14735. 2000.

• Molecular signaling mechanisms of migration and invasion
  Stupack, D.G., Cho, S.Y. & Klemke, R.L.. Imm. Res. 121:83-88. 2000.

• Extracellular-regulated kinase activation and CAS/Crk coupling regulate cell migration and suppress apoptosis during invasion of the extracellular matrix.
  Cho, S.Y. & Klemke, R.L.. J. Cell. Biol. 149(1):223-236. 2000.

• ErbB-2 is necessary for induction of carcinoma cell invasion by erbB family receptor tyrosine kinases.
  Spencer, K., Graus-Porta, D., Leng, J., Hynes, N. & Klemke, R.L.. J. Cell. Biol. 148(4):385-397. 2000.

• Potentiation of cell migration by adhesion-dependent cooperative signals from the GTPase Rac and Raf kinase
  Leng, J., Klemke, R.L., Reddy, A.C. & Cheresh, D.A. . J. Biol. Chem. 274:37855-37861. 1999.

• Regulation of contraction and membrane ruffling by distinct signals in migratory cells.
  Cheresh, D.A., Leng, J. & Klemke, R.L. J. Cell. Biol. 146:1107-1116

• Four human Ras homologs differ in their abilities to activate Raf-1, induce transformation and stimulate cell motility.
  Voice, J.K., Klemke, R.L., Le, A. & Jackson, J.H.. J. Biol. Chem. 274:17164-17170. 1999.

• Integrin requirement for sustained MAP kinase activity during angiogenesis.
  Eliceiri, B.P., Klemke, R.L., Stromblad, S. & Cheresh, D.A.. J. Cell. Biol. 140(5):1255-1263. 1998.

• CAS/Crk coupling serves as a "molecular switch" for induction of cell migration.
  Klemke, R.L., Leng, J., Molander, R., Brooks, P.C., Vuori, K. & Cheresh, D.A.. J. Cell. Biol. 140(4):961-972. 1998

• Adenovirus endocytosis and cell motility require distinct signaling pathways mediated by av integrins
  Li, E., Stupack, D., Klemke, R.L, Cheresh, D.A. & Nemerow, G.R.. J. Virology 7:2055-2061. 1998.

• Insulin-like growth factor receptor cooperates with integrin avb5 to promote tumor cell dissemination in vivo.
  Brooks, P.C., Klemke, R.L., Schon, S., Lewis, J.M., Schwartz, M.A. & Cheresh, D.A.J. Cell. Invest. 99(6):1390-1398. 1997

• Regulation of cell motility by mitogen-activated protein kinase.
  Klemke, R.L., Cai, S., Giannini, A.L., Gallagher, P.J., de Lanerolle, P. & Cheresh, D.A. J. Cell. Biol. 137(2):481-492. 1997.

• Anti-integrin avb3 blocks human breast cancer growth and angiogenesis in human skin.
  Brooks, P.C., Stromblad, S., Klemke, R.L., Visscher, D., Sarkar, F.H. & Cheresh, D.A. J. Clin. Invest. 96:1815-1822. 1995.

• Receptor tyrosine kinase signaling required for integrin avb5-directed cell motility but not adhesion on vitronectin.
  Klemke, R.L., Yebra, M., Bayna, E.M. & Cheresh, D.A. J. Cell. Biol. 127(3):850-866. 1994.

• Comparison of the ontogeny of specific cell surface determinants on normal and delayed implanting mouse embryos.
  Klemke, R.L. & Weitlauf, H.M. J. Repro. Fert. 99:167-172. 1992.

• The effects of THC on macromolecule synthesis in preimplantation mouse embryos
  Klemke, R.L. & Rogers, S.H. . Res. Comm. in Subs. of Abuse. 11(4):207-210. 1990.